Centre for Genetic Physiology

Centre for Genetic Physiology

The core facility/platform provides resources and service for physiological characterization of normal and genetically modified mice. A focus is on cardiovascular studies of living conscious animals and on in vitro examination of isolated cardiac and vascular tissue.

Description

Karolinska Institutet Core Facility for Physiological Phenotyping focuses on methods to characterize genetically modified mice.

Infrastructure/Methods

In vivo models

Mouse telemetry
A transmitter is implanted under the skin or into the abdomen, whereupon blood pressure, ECG, body temperature, and locomotor activity could be registered continuously in the conscious, freely moving animal for up to three months.

In vivo blood pressure
Blood pressure can be measured with telemetry, as described above, or by means of a swivel system with a catheter in the animal’s carotid artery. In the latter system, drugs could also be administered by inserting a second catheter in a vein. Furthermore, blood pressure could be measured in anaesthetized mice by using a sensitive pressure sensor (Samba sensor). With this technique, the augmentation index (AIX) could be calculated which provides further information on the condition of the cardiovascular system.

Somedic INCA system
This is a cage for whole animal metabolism in mice where the animal’s O2 consumption, CO2 production, core temperature, heart rate and activity are registered. The temperature of the box could be changed, thus allowing challenges of the metabolism. The system can be combined with exercise (running-wheel).

Mouse echocardiography
A high resolution ultrasound system (15-Mhz probe, Phillips ATL, with Doppler) can be used to evaluate left ventricular function, aortic or mitral valve flow, as in humans, with calculations of ejection fraction, wall thickening etc.

Ex vivo models

Isolated heart model
The isolated, modified Langendorff-setup is established.

Studies on isolated tissue
An organ bath system with 4 chambers for studies of vessel reactivity in arterial/venous rings is available. Furthermore, we have techniques for studies of paced cardiac trabecular preparations, isolated cardiomyocytes, electrophysiology and intracellular calcium measurements.

Practical information

We can either assist you in your experiments or let you use the equipment if you have sufficient skills. Please contact us for more information.

Interested in Industry collaboration
Karolinska Institutet
Department of Physiology and Pharmacology
171 77 Stockholm
+46 (0)8 524 822 70