Liver Cell Laboratory - Primary Hepatocytes
Our research team is experienced in the isolation and transplantation of hepatocytes to treat liver disease, and we bring this experience to provide hepatocytes for basic research from liver tissue not suited for clinical transplantation such as tissue obtained from patients undergoing liver resection for cancer. Our goal is to share these primary human liver cells with other investigators and provide them with primary human hepatocytes for research. It is also important for us that all of the cells we isolate come to use. Primary liver cells are the only in vitro system for liver studies that maintain the advanced functions of an adult hepatocyte. Under special culturing conditions these cells preserve their differentiated phenotype but do not divide. Using cultured primary hepatocytes one can study a wide range of systems from pharmaceutical metabolism, toxicity, synthesis and metabolism to advanced canalicular transport. The application of primary hepatocytes is vast and also includes bioreactors and transplantation studies. We collaborate with and provide cells to a growing list of research groups at Karolinska Institutet, SWE-Tox, Uppsala University, AstraZeneca and internationally to Norway and the Netherlands.
While our service focus on providing primary hepatocytes in suspension or plated on culture plates or flasks, other cell types from the liver can also be provided. In addition, we offer education of staff and scientists in isolation technique and the handling and culture of the cells we provide. We also offer advice on experimental design and troubleshooting problems that may occur.
There are requirements to receive liver hepatocytes and hence before you can receive cells from our facility, we need to have an ethical permit and a transfer agreement in place. Users will have to arrange and pay for transportation of the cells and a service fee will be charged.
Please visit our website for the latest price information and contact Dr. Ewa Ellis for more information or to set up an agreement.