Swedish Human Proteome Resource (HPR)
We perform a systematic exploration of the human proteome with Affinity Proteomics, combining high-throughput generation of affinity-purified (mono-specific) antibodies with protein profiling using tissue arrays. A Protein Atlas has been created to show the expression and localization of proteins in a large variety of normal human tissues and cancer cells.
The Swedish Human Proteome Resource (HPR) program, funded by the Knut and Alice Wallenberg Foundation, has been set-up to allow the systematic exploration of the human proteome with Affinity (Antibody) Proteomics, combining high-throughput generation of affinity-purified (mono-specific) antibodies with protein profiling using tissue arrays.
The basic concept of the resource centre is to produce specific antibodies to human target proteins using a high-throughput method involving the cloning and protein expression of Protein Epitope Signature Tags (PrESTs). The antibodies are subsequently used for functional analysis of the corresponding proteins in a wide range of assay platforms.
A protein atlas has been created to show the expression and localization of proteins in a large variety of normal human tissues and cancer cells. The data is presented as high resolution images representing immunohistochemically stained tissue sections. Available proteins (genes) can be reached through a specific search on our website (by gene/protein name/id or classification, such as kinase or protease) or by browsing the individual chromosomes.
We have recently described a standardized approach for generating recombinant proteins from coding sequences identified from the human genome sequence. This approach has been further developed with regard to
- bioinformatics algorithms that have allowed the identification of coding sequences that are amenable both to recombinant protein expression and the generation of specific antibodies,
- a robust E. coli recombinant protein expression system,
- systematic antibody production associated with antigen purification of polyclonal sera and,
- systematic approaches to utilize these reagents, in particular in tissue arrays.
These methodologies are all developed to allow high throughput on a genome wide scale. The vision is to enable the systematic generation of quality assured antibodies to all non-redundant human proteins and to use these reagents to functionally explore human proteins, protein variants and protein interactions.
Visit our website for more information.