Swedish Human Proteome Resource (HPR)

Swedish Human Proteome Resource (HPR)

The Swedish Human Proteome Resource (HPR) has been set-up to allow the systematic exploration of the human proteome with Antibody Proteomics, combining high-throughput generation of affinity-purified antibodies with protein profiling using tissue arrays.

Description

We perform a systematic exploration of the human proteome with Affinity Proteomics, combining high-throughput generation of affinity-purified (mono-specific) antibodies with protein profiling using tissue arrays. A Protein Atlas has been created to show the expression and localization of proteins in a large variety of normal human tissues and cancer cells.

The Swedish Human Proteome Resource (HPR) program, funded by the Knut and Alice Wallenberg Foundation, has been set-up to allow the systematic exploration of the human proteome with Affinity (Antibody) Proteomics, combining high-throughput generation of affinity-purified (mono-specific) antibodies with protein profiling using tissue arrays.

The basic concept of the resource centre is to produce specific antibodies to human target proteins using a high-throughput method involving the cloning and protein expression of Protein Epitope Signature Tags (PrESTs). The antibodies are subsequently used for functional analysis of the corresponding proteins in a wide range of assay platforms.

A protein atlas has been created to show the expression and localization of proteins in a large variety of normal human tissues and cancer cells. The data is presented as high resolution images representing immunohistochemically stained tissue sections. Available proteins (genes) can be reached through a specific search on our website (by gene/protein name/id or classification, such as kinase or protease) or by browsing the individual chromosomes.

Infrastructure/Methods

We have recently described a standardized approach for generating recombinant proteins from coding sequences identified from the human genome sequence. This approach has been further developed with regard to

  • bioinformatics algorithms that have allowed the identification of coding sequences that are amenable both to recombinant protein expression and the generation of specific antibodies,
  • a robust E. coli recombinant protein expression system,
  • systematic antibody production associated with antigen purification of polyclonal sera and,
  • systematic approaches to utilize these reagents, in particular in tissue arrays.

These methodologies are all developed to allow high throughput on a genome wide scale. The vision is to enable the systematic generation of quality assured antibodies to all non-redundant human proteins and to use these reagents to functionally explore human proteins, protein variants and protein interactions.

Practical information

Visit our website for more information.

Interested in Industry collaboration
AlbaNova University Center
Department of Proteomics (School of Biotechnology)
106 91 Stockholm
+46 (0)8 5537 8403